The new edition of this successful textbook explains the basic principles behind the key techniques currently used in the modern biochemical laboratory in a way that is comprehensive and approachable for students who are not physical chemists. It bridges the gap between general biochemistry textbooks and the more specialist books covering individual techniques. The second edition will maintain the strengths of the first and include the following specific changes: Incorporate much more material on proteomics. Include more information on small-scale physical methods and fourier transform mass spectrometry Cover the very latest developments in biological NMR Revise the chapter on chromatography - to make it shorter Provide some case studies to help readers appreciate the multi-disciplinary nature of physical biochemistry Update of the bibliography.