Based on a course the author teaches, this is a comprehensive book that discusses physicochemical and biomimetic properties for lead optimization in drug discovery. It begins with an introduction to the drug discovery process and basic pharmacokinetic properties like absorption and distribution in vivo. This book uniquely focuses on chromatography as a measuring technique and covers its principles, advantages and applications. It discusses methods for measuring physicochemical properties and how they influence absorption and distribution and includes new approaches and techniques to measure these properties. The author uses real-life examples to present how the measured biomimetic chromatographic descriptors work in shaping drug distribution and how to apply those parameters for successful drug candidates. Each chapter concludes with a series of questions, which have answers in the Appendix, and also makes lecture slides available via the book's wiley.com page for adopters to access. Additionally, enhanced ebook versions contain video clips that show experimental procedures and explain difficult concepts.